Yellow box frame in olympus viewer 36/3/2023 We demonstrate the speed, field of view, resolution and versatility of our instrument by imaging various systems, including Drosophila egg chamber development, zebrafish whole-brain activity and zebrafish embryonic development – up to nine embryos at a time. Our instrument achieves a resolution of 450 nm laterally and 2 μm axially over an imaging volume of 3,000 × 800 × 300 μm. We present DaXi, a single-objective light-sheet microscope design based on oblique plane illumination that achieves: (1) a wider field of view and high-resolution imaging via a custom remote focusing objective (2) fast volumetric imaging over larger volumes without compromising image quality or necessitating tiled acquisition (3) fuller image coverage for large samples via multi-view imaging and (4) higher throughput multi-well imaging via remote coverslip placement. Movie quality: YCbCr 4:2:2 / Frame rate: same as movie setting (24p, 30p and 60p settings are not recommended.The promise of single-objective light-sheet microscopy is to combine the convenience of standard single-objective microscopes with the speed, coverage, resolution and gentleness of light-sheet microscopes. GUI can be hidden on the connected device by pressing down on INFO button for recording an external drive under the following condition.With (GUI* is shown on connected device only, and peaking function is not available when connected by HDMI.) Rec Run / Free Run, Drop Frame / Non-drop Frame Lens-IS priority is available.Ĥ types of grid lines / 4 colours for focus peaking M-IS2(Image sensor shift with multi motion IS), Off M-IS1(Image sensor shift and electronic image stabiliser with multi motion IS), (Further continued shooting possible by pressing movie record button again) Mode 1 is available when recording framrate is less than 30p.ġ, 2, 4, 8 sec.×4 (Can be activated only in Movie Mode with Movie Effect on)ġ280 × 720(HD) / 5 fps, 10 fps, 15 fps, 30 fps One-shot Echo / Multi-echo / Art Fade / Old Film Excluding when particular Art Filters are used.UHS-II or UHS-I U3 card is recommended for ALL-I shooting.Class 10 or higher SD card is recommended for shooting movies.Frame rates may drop when particular Art Filters and Movie Effect are used.Locks with a half-press of the shutter button (Can be set to Fn button as well) HDR Shooting, monitor and EVF displays only up to ☓ EV.Still photo ±5 EV, movie ☓ EV, with selectable EV adjustment steps (1/3, 1/2, 1) Manual ISO : Low (approx.100), 200 - 25600, 1/3 or 1 EV steps selectable Portrait, e-Portrait, Landscape, Landscape + Portrait, Sport, Hand-held Starlight, Night scene, Night + Portrait, Children, High Key, Low Key, DIS mode, Macro, Nature Macro, Candle, Sunset, Documents, Panorama, Fireworks, Beach & Snow, Fisheye Conv., Wide Conv., Macro Conv.,Panning, 3D ,ġ 8 images automatically shot and combined into a single image compositeĢ For 3D lens(H-FT012 by Panasonic) only, still photo onlyĪuto ISO : Low (approx. My set can be set to mode dial function.Selectable from menu as a function on Fn button.S: Shutter priority AE, M: Manual, Bulb, Time, Scene select AE, Art Filter, I Auto, P: Program AE (Program shift can be performed), A: Aperture priority AE, Activate/deactivate with double tap on rear monitorįocus ring rotation activates Live View image magnification or focus peaking when in S-AF+MF or MF modeĬhange peaking settings using the Info buttonĬolour selection: white, black, red, yellowĭigital ESP metering (324-area multi pattern metering), Centre-weighted average metering, Spot metering, Spot metering with highlight control, Spot metering with shadow control.
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